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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16152 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepM2-AH
- Backbone size w/o insert (bp) 10000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMus musculus potassium large conductance calcium-activated channel
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Alt namemSlo1
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Alt nameGeneID:16531
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Alt nameMGI:99923
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SpeciesM. musculus (mouse)
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Insert Size (bp)4740
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MutationN/A
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GenBank IDNM_010610
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Entrez GeneKcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pM2AH + mSlo1 was a gift from Larry Salkoff (Addgene plasmid # 16152 ; http://n2t.net/addgene:16152 ; RRID:Addgene_16152) -
For your References section:
mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels. Butler A, Tsunoda S, McCobb DP, Wei A, Salkoff L. Science. 1993 Jul 9. 261(5118):221-4. 10.1126/science.7687074 PubMed 7687074
Map uploaded by the depositor.