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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepM2-AH
- Backbone size w/o insert (bp) 10000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecalcium-activated potassium channel
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Alt nameAAA92290
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SpeciesH. sapiens (human), M. musculus (mouse)
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Insert Size (bp)3900
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MutationN/A
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GenBank IDU23767
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Entrez GeneKCNMA1 (a.k.a. BKTM, CADEDS, IEG16, KCa1.1, LIWAS, MaxiK, PNKD3, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, hSlo, mSLO1)
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Entrez GeneKcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results do not exactly match the above GenBank accession numbers U23767 (nucleotide) and AAA92290 (protein) due to the cloning strategy employed for this plasmid. The beginning of the insert sequence from the Hind3 site (AAGCTT) to the Mlu1 site (ACGCGT) is from a mouse cDNA that contains an N-terminus that is highly conserved but not identical to that of the human channel. Prior to the starting Met of Slo1, this plasmid contains a short sequence from the mouse Slo1 5' untranslated region (5'UTR).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pM2AH + Slo1 was a gift from Larry Salkoff (Addgene plasmid # 16195 ; http://n2t.net/addgene:16195 ; RRID:Addgene_16195) -
For your References section:
A human calcium-activated potassium channel gene expressed in vascular smooth muscle. McCobb DP, Fowler NL, Featherstone T, Lingle CJ, Saito M, Krause JE, Salkoff L. Am J Physiol. 1995 Sep . 269(3 Pt 2):H767-77. 10.1152/ajpheart.1995.269.3.H767 PubMed 7573516