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Addgene

pM2AH + Slo1
(Plasmid #16195)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16195 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pM2-AH
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    calcium-activated potassium channel
  • Alt name
    AAA92290
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    3900
  • Mutation
    N/A
  • GenBank ID
    U23767
  • Entrez Gene
    KCNMA1 (a.k.a. BKTM, CADEDS, IEG16, KCa1.1, LIWAS, MaxiK, PNKD3, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, hSlo, mSLO1)
  • Entrez Gene
    Kcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results do not exactly match the above GenBank accession numbers U23767 (nucleotide) and AAA92290 (protein) due to the cloning strategy employed for this plasmid. The beginning of the insert sequence from the Hind3 site (AAGCTT) to the Mlu1 site (ACGCGT) is from a mouse cDNA that contains an N-terminus that is highly conserved but not identical to that of the human channel. Prior to the starting Met of Slo1, this plasmid contains a short sequence from the mouse Slo1 5' untranslated region (5'UTR).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM2AH + Slo1 was a gift from Larry Salkoff (Addgene plasmid # 16195 ; http://n2t.net/addgene:16195 ; RRID:Addgene_16195)
  • For your References section:

    A human calcium-activated potassium channel gene expressed in vascular smooth muscle. McCobb DP, Fowler NL, Featherstone T, Lingle CJ, Saito M, Krause JE, Salkoff L. Am J Physiol. 1995 Sep . 269(3 Pt 2):H767-77. 10.1152/ajpheart.1995.269.3.H767 PubMed 7573516

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