pAAV-CAG-ArchT-GFP
(Plasmid #29777)

• Purpose: AAV-mediated expression of ArchT-GFP for neuronal inhibition
• Depositing Lab: Edward Boyden
• Publication: Han et al Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13.

Backbone

• Vector backbone: AAV with CAG promoter
• Backbone manufacturer: Scott Sternson
• Backbone size w/o insert (bp): 5451
• Vector type: Mammalian Expression, AAV ; Adeno-associated virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Use recombinase-free E. coli (Stbl3, XL-10, Sure2 et.al), grow at 30C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ArchT-GFP
• Alt name: archaerhodopsin
• Alt name: archaerhodopsin TP009
• Alt name: ArchT
• Species: H. strain TP009
• Insert Size (bp): 744
• Mutation: N/A
• Promoter: CAG
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: gctctagagcctctgctaacc
• 3′ sequencing primer: gcagcgtatccacatagcg

Resource Information

• Articles Citing this Plasmid:
  • 21 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.