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pTH385-GST-TEV-SUP35
(Plasmid #29744)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29744 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4954
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For expression, BL21 at 25 C works well.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUP35 gene encoding translation release factor 3 from S. cerevisiae
  • Alt name
    eRF3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2086
  • Mutation
    wild type
  • GenBank ID
    NM_001180479
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GST5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH385-GST-TEV-SUP35 was a gift from Tobias von der Haar (Addgene plasmid # 29744 ; http://n2t.net/addgene:29744 ; RRID:Addgene_29744)
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