pCS2 GFP-GSK3-MAPK
(Plasmid #29689)

• Purpose: GFP reporter protein fused to three GSK3 phosphorylation sites
• Depositing Lab: Edward De Robertis
• Publication: Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4096
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP glycogen synthase kinase 3 recognition site primed by MAPK
• Alt name: GFP fused to 3x GSK3 phosphorylation sites
• Insert Size (bp): 121
• Mutation: recognition sites only, and E3 ligase site
• Promoter: SP6
• Tags / Fusion Proteins:
  • Flag-EGFP (N terminal on backbone)
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This artificial GFP-GSK3-MAPK construct was generated by adding to the C terminus of GFP synthetic oligonucleotides encoding for AAASAAASAAASAPASPAAAAAAAAPPAYDYKDDDDK. See Figure 6A of the associated publication for more details.