pOSCAR
(Plasmid #29639)

• Purpose: (Empty Backbone)
• Depositing Labs: David_L Andrews, Scott Gold
• Publication: Paz et al Fungal Genet Biol. 2011 Mar 5. ():.

Backbone

• Vector backbone: pPZP-RCS2
• Backbone manufacturer: Stanton Gelvin

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Growth instructions: DB3.1 (ccdB resistant)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Alt name: OSCAR
• GenBank ID: HM623914

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

To constuct this vector the 2.5 kb Gateway cassette attP2R-ccdB-cmR-attP3 was cloned into pCR2.1-TOPO (Invitrogen) generating plasmid pTOPO-attP2R-ccdB-attP3. The P2R-ccdB-cmR-P3 cassette was then excised from pTOPO-attP2R-ccdB-attP3 by digestion with HindIII and KpnI and inserted in the binary vector pPZP-RCS2.

Note that Addgene's sequencing results contain a single nucleotide mismatch at bp#4262 when compared to the depositor's sequence. This discrepancy does not affect the function of the plasmid.