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Addgene

pDONRCBX
(Plasmid #29634)

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Item Catalog # Description Quantity Price (USD)
Plasmid 29634 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR201
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 4470
  • Vector type
    Gateway donor

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    DB3.1 (ccdB resistant)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    DelsGate
  • GenBank ID
    EU360889

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a selectable marker for U. maydis transformation and was constructed by inserting a ~2.3 kb EcoICRI carboxin
resistance (cbxR) conferring fragment from pGR3 (kindly provided by J. Hargreaves) into the blunt ended NspI site of pDONR201.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONRCBX was a gift from David_L Andrews & Scott Gold (Addgene plasmid # 29634 ; http://n2t.net/addgene:29634 ; RRID:Addgene_29634)

  • For your References section:

    DelsGate, a robust and rapid gene deletion construction method. Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold SE. Fungal Genet Biol. 2008 Apr . 45(4):379-88. 10.1016/j.fgb.2007.11.001 PubMed 18248826
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