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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDsRed1-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCent2
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Alt nameCentrin 2
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SpeciesH. sapiens (human)
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Entrez GeneCETN2 (a.k.a. CALT, CEN2)
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Tag
/ Fusion Protein
- dsRed (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer DsRed1-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dsRed-cent2 was a gift from Joseph Gleeson (Addgene plasmid # 29523 ; http://n2t.net/addgene:29523 ; RRID:Addgene_29523) -
For your References section:
Lis1 and doublecortin function with dynein to mediate coupling of the nucleus to the centrosome in neuronal migration. Tanaka T, Serneo FF, Higgins C, Gambello MJ, Wynshaw-Boris A, Gleeson JG. J Cell Biol. 2004 Jun 7. 165(5):709-21. 10.1083/jcb.200309025 PubMed 15173193