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Addgene

pJAK1.A
(Plasmid #29460)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29460 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMV361
  • Backbone size (bp) 4445
  • Vector type
    Bacterial Expression ; Mycobacteria expression
  • Promoter Hsp60

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdb survival
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Integrative mycobacterial destination vector for recombination cloning.

Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJAK1.A was a gift from Zakaria Hmama (Addgene plasmid # 29460 ; http://n2t.net/addgene:29460 ; RRID:Addgene_29460)

  • For your References section:

    A broad-range of recombination cloning vectors in mycobacteria. Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z. Plasmid. 2009 Nov . 62(3):158-65. 10.1016/j.plasmid.2009.07.003 PubMed 19647016
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