SEMA3D (mouse)
(Plasmid
#29452)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29452 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCRII
- Backbone size w/o insert (bp) 3900
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Vector typefor ISH
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSEMA3D
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Alt namecollapsin 2
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Alt namesemaphorin 3D
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SpeciesM. musculus (mouse)
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Insert Size (bp)6000
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Mutationinsert is not full length; contains multiple point mutations
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GenBank IDNM_028882
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Entrez GeneSema3d (a.k.a. 4631426B19Rik)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this vector was designed to generate a probe for in situ hybridization (ISH) and is functional for this purpose. The SEMA3D sequence is not full-length and may contain additional mutations when compared to the GenBank reference sequence.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SEMA3D (mouse) was a gift from Jonathan Raper (Addgene plasmid # 29452 ; http://n2t.net/addgene:29452 ; RRID:Addgene_29452)