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Addgene

SEMA3A (mouse) pBLUE
(Plasmid #29450)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29450 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBLUESCRIPT (SK)
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    for ISH

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEMA3A
  • Alt name
    Collapsin 1
  • Alt name
    Semaphorin 3A
  • Alt name
    Collapsin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2900
  • Mutation
    G25R, S67R and Y124C confirmed
  • GenBank ID
    NM_009152
  • Entrez Gene
    Sema3a (a.k.a. Hsema-I, SEMA1, SemD, Semad, coll-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this vector was designed to generate a probe for in situ hybridization (ISH) and is functional for this purpose. The SEMA3A sequence has not been verified to be full-length and may contain additional mutations when compared to the GenBank reference sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SEMA3A (mouse) pBLUE was a gift from Jonathan Raper (Addgene plasmid # 29450 ; http://n2t.net/addgene:29450 ; RRID:Addgene_29450)
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