Skip to main content
Addgene

AP-SEMA3C (chick)
(Plasmid #29449)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29449 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAG
  • Backbone manufacturer
    modified from PCDNA3 see methods in associated publication.
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP-SEMA3C
  • Alt name
    Collapsin 3
  • Alt name
    Sema 3c
  • Alt name
    Semaphorin 3C
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    3600
  • GenBank ID
    NM_204243
  • Entrez Gene
    SEMA3C
  • Tag / Fusion Protein
    • AP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer TTC GGC TTC TGG CGT GTG ACC
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AP-SEMA3C (chick) was a gift from Jonathan Raper (Addgene plasmid # 29449 ; http://n2t.net/addgene:29449 ; RRID:Addgene_29449)

  • For your References section:

    Secreted chick semaphorins bind recombinant neuropilin with similar affinities but bind different subsets of neurons in situ. Feiner L, Koppel AM, Kobayashi H, Raper JA. Neuron. 1997 Sep . 19(3):539-45. 10.1016/S0896-6273(00)80370-0 PubMed 9331347
Related items:
Commonly requested with: