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Addgene

pSVL(D3)
(Plasmid #29335)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29335 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSVL
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4896
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Trimer of the HDV genome
  • Species
    Hepatitis Delta Virus
  • Insert Size (bp)
    5037

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EBV-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSVL(D3) was a gift from John Taylor (Addgene plasmid # 29335 ; http://n2t.net/addgene:29335 ; RRID:Addgene_29335)

  • For your References section:

    Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen. Kuo MY, Chao M, Taylor J. J Virol. 1989 May . 63(5):1945-50. PubMed 2649689
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