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Addgene

CALNL-DCX-eGFP
(Plasmid #28310)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28310 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CALNL-eGFP
  • Backbone manufacturer
    Addgene plasmid 13770
  • Backbone size w/o insert (bp) 4698
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Doublecortin
  • Alt name
    DCX
  • Alt name
    pCALNL-DCX
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Dcx
  • Tag / Fusion Protein
    • eGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CALNL-DCX-eGFP was a gift from Joseph Loturco (Addgene plasmid # 28310 ; http://n2t.net/addgene:28310 ; RRID:Addgene_28310)

  • For your References section:

    Dcx reexpression reduces subcortical band heterotopia and seizure threshold in an animal model of neuronal migration disorder. Manent JB, Wang Y, Chang Y, Paramasivam M, LoTurco JJ. Nat Med. 2009 Jan . 15(1):84-90. 10.1038/nm.1897 PubMed 19098909
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