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Addgene

pGal4AD-CIBN
(Plasmid #28246)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28246 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGADT7rec
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIB1 (1-171aa)
  • Alt name
    CIBN
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    505
  • Tag / Fusion Protein
    • Gal4AD (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGal4AD-CIBN was a gift from Chandra Tucker (Addgene plasmid # 28246 ; http://n2t.net/addgene:28246 ; RRID:Addgene_28246)

  • For your References section:

    Rapid blue-light-mediated induction of protein interactions in living cells. Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL. Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524 10.1038/nmeth.1524 PubMed 21037589
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