pGal4BD-CRY2PHR
(Plasmid #28244)

• Purpose: Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation
• Depositing Lab: Chandra Tucker
• Publication: Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 7480
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cry2
• Alt name: cryptochrome 2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1507
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Tag / Fusion Protein:
  • Gal4BD (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry