pEGFP-C1-ER alpha
(Plasmid #28230)

• Purpose: Mammalian expression of Estrogen Receptor alpha fused to EGFP
• Depositing Lab: Michael Mancini
• Publication: Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha, XL10G
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Estrogen Receptor alpha
• Species: H. sapiens (human)
• Insert Size (bp): 1789
• GenBank ID: NM_000125
• Entrez Gene: ESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: EGFP-N

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: pRST7-ER alpha; Carolyn Smith, Baylor College of Medicine (originally from Donald P. McDonnell)
• Articles Citing this Plasmid:
  • 22 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tzukerman MT, Esty A, Santiso-Mere D, Danielian P, Parker MG, Stein RB, Pike JW, McDonnell DP 1994 Human estrogen receptor transactivational capacity is determined by both cellular and promoter context and mediated by two functionally distinct intramolecular regions. Mol Endocrinol 8:21–30