SI4-Jamc.2
(Plasmid #28216)

• Depositing Lab: Gavin Wright
• Publication: Crosnier et al BMC Biol. 2010 . 8():76.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 5925
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Jamc.2 mouse monoclonal antibody
• Alt name: jamc.2
• Species: D. rerio (zebrafish)
• Entrez Gene: jam3a (a.k.a. jamc.2, si:ch211-277g22.3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13_puc_Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See map and Genbank file for plasmid details.

Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 3370-3371 and 3380-3382 when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.