pEB-C5
(Plasmid #28213)

• Purpose: 5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit
• Depositing Lab: Linzhao Cheng
• Publication: Chou et al Cell Res. 2011 Jan 18. ():.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 10200
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: STBL3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Oct4, Sox2, Klf4, c-Myc, Lin28
• Species: M. musculus (mouse)
• Insert Size (bp): 7612
• Entrez Gene: Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)
• Entrez Gene: Sox2 (a.k.a. Sox-2, lcc, ysb)
• Entrez Gene: Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
• Entrez Gene: Klf4 (a.k.a. EZF, Gklf, Zie)
• Entrez Gene: Lin28a (a.k.a. Gm10299, Lin-28, Lin28, Tex17, lin-28A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results using the CMV-F and pCEP-F primers found 2 sequence variants within and after the CMV enhancer. One is a C->G mismatch at position 775 within the CMV enhancer and the second is a deletion at position 949 just after the end of the CAG enhancer, as compared to the full plasmid sequence provided by the depositing scientist. The significance of the two sequence variants is unclear; however, the plasmid functions as described in the associated publication. Addgene's other 4 sequencing results completely matched the depositor's sequence.