Skip to main content
Addgene

UBH14
(Plasmid #28158)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28158 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pET28a-LIC (Addgene plasmid 26094)
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7328
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E3 ligase UHRF2
  • Alt name
    PDB: 3OLN
  • Alt name
    E3 ligase UHRF2, SRA domain
  • Alt name
    UBH14
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    687
  • Mutation
    SRA Domain (residues 419-648)
  • Entrez Gene
    UHRF2 (a.k.a. NIRF, RNF107, TDRD23, URF2)
  • Tag / Fusion Protein
    • His tag with thrombin cleavage (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ligation-independent cloning (unknown if destroyed)
  • 3′ cloning site ligation-independent cloning (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UBH14 was a gift from Cheryl Arrowsmith (Addgene plasmid # 28158 ; http://n2t.net/addgene:28158 ; RRID:Addgene_28158)
Related items:
Commonly requested with: