AAV-CAG-hChR2_tdTomato
(Plasmid #28015)

• Purpose: Expresses humanized ChR2 fused to tdTomato for optogenetic activation
• Depositing Lab: Karel Svoboda
• Publication: Mao et al Neuron. 2011 Oct 6;72(1):111-23.

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Backbone

• Vector backbone: AAV2/1
• Backbone size w/o insert (bp): 4883
• Vector type: AAV ; adeno associate viral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: humanized ChR2-tdTomato
• Alt name: hChR2-tdTomato
• Insert Size (bp): 2363
• Promoter: CAG
• Tag / Fusion Protein:
  • tdTomato

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: TCGGCTTCTGGCGTGTGACCGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

with ITRs, this vector is easy to have recombination. Check with multiple restriction digestions.