CAV1-K*R-mEGFP
(Plasmid #27766)

• Depositing Lab: Ari Helenius
• Publication: Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Backbone

• Vector backbone: pmEGFP-N1
• Backbone manufacturer: J. Lippincott-Schwartz
• Backbone size w/o insert (bp): 4695
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: caveolin-1, lysine null mutant
• Alt name: CAV1
• Alt name: cav-1
• Species: H. sapiens (human)
• Insert Size (bp): 545
• Mutation: All lysines mutated to arginines (K*R)
• GenBank ID: NM_001753
• Entrez Gene: CAV1 (a.k.a. BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21)
• Tag / Fusion Protein:
  • monomeric EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Foward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mutant CAV1-K*R cannot be ubiquitinated as all lysines are mutated to arginines.