CAV1-mEGFP-mCherry
(Plasmid #27763)

• Depositing Lab: Ari Helenius
• Publication: Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5069
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: caveolin-1
• Alt name: CAV1
• Alt name: cav-1
• Species: Canis lupus familiaris
• Insert Size (bp): 2034
• GenBank ID: NM_001003296
• Entrez Gene: CAV1
• Tag / Fusion Protein:
  • monomeric EGFP-monomeric Cherry (tandem) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pcDNA5/FRT/TO backbone is compatible with Flp-In recombination for generation of stable cell lines and/or tet-inducible expression. Can also be used for transient expression.