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Addgene

cavin-3-HA
(Plasmid #27711)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27711 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHA-N-DEST
  • Backbone manufacturer
    Helenius lab
  • Backbone size w/o insert (bp) 5502
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cavin-3
  • Alt name
    SRBC
  • Alt name
    PRKCDBP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    783
  • Mutation
    R8P
  • GenBank ID
    NM_145040
  • Entrez Gene
    CAVIN3 (a.k.a. HSRBC, PRKCDBP, SRBC, cavin-3)
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attL1/attR1 (destroyed during cloning)
  • 3′ cloning site attL2/attR2 (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mutation R8P is a known natural variant of cavin-3 (http://www.uniprot.org/uniprot/Q969G5) The cavin-3 cDNA used in this plasmid originated from the ORFeome library.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cavin-3-HA was a gift from Ari Helenius (Addgene plasmid # 27711 ; http://n2t.net/addgene:27711 ; RRID:Addgene_27711)

  • For your References section:

    Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes. Hayer A, Stoeber M, Bissig C, Helenius A. Traffic. 2010 Mar . 11(3):361-82. 10.1111/j.1600-0854.2009.01023.x PubMed 20070607
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