cavin-2-mEGFP
(Plasmid #27710)

• Depositing Lab: Ari Helenius
• Publication: Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5069
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cavin-2
• Alt name: SDPR
• Alt name: SDR
• Species: H. sapiens (human)
• Insert Size (bp): 2027
• GenBank ID: NM_004657
• Entrez Gene: CAVIN2 (a.k.a. PS-p68, SDPR, SDR, cavin-2)
• Tag / Fusion Protein:
  • monomeric EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry