cavin-1-mEGFP
(Plasmid #27709)

• Depositing Lab: Ari Helenius
• Publication: Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5069
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cavin-1
• Alt name: PTRF, cavin
• Species: M. musculus (mouse)
• Insert Size (bp): 1929
• GenBank ID: NM_008986
• Entrez Gene: Cavin1 (a.k.a. 2310075E07Rik, Cav-p60, Cavin, Ptrf)
• Tag / Fusion Protein:
  • monomeric EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry