CAV1-deltaN-mEGFP
(Plasmid #27707)

• Depositing Lab: Ari Helenius
• Publication: Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3961
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: caveolin-1, N-terminal truncated (60-178)
• Alt name: CAV1, cav-1
• Species: Canis lupus familiaris
• Insert Size (bp): 1109
• Mutation: N-terminal truncation with AA 1-59 missing. The resulting sequence is CAV1-(60-178).
• GenBank ID: NM_001003296
• Entrez Gene: CAV1
• Tag / Fusion Protein:
  • monomeric EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: ACC TCT ACA AAT GTG GTA TGG CTG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This N-terminally truncated CAV1 forms 8S, but not 70S caveolar complexes. This results in delivery of unassembled CAV1 to the plasma membrane.