CAV1-mEGFP
(Plasmid #27704)

• Depositing Lab: Ari Helenius
• Publication: Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 3961
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: caveolin-1
• Alt name: CAV1, cav-1
• Species: Canis lupus familiaris
• Insert Size (bp): 1322
• GenBank ID: NM_001003296
• Entrez Gene: CAV1
• Tag / Fusion Protein:
  • monomeric EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: ACC TCT ACA AAT GTG GTA TGG CTG

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry