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Addgene

pHZ081
(Plasmid #27506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 6167
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pcol-10::lin-4::let-858_3'UTR
  • Alt name
    lin-4
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    90
  • Entrez Gene
    lin-4 (a.k.a. CELE_F59G1.6)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are some discrepancies between Addgene's quality controls sequence and the depositor's sequence, but the Fire lab does not believe they will affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHZ081 was a gift from Andrew Fire (Addgene plasmid # 27506 ; http://n2t.net/addgene:27506 ; RRID:Addgene_27506)

  • For your References section:

    Cell autonomous specification of temporal identity by Caenorhabditis elegans microRNA lin-4. Zhang H, Fire AZ. Dev Biol. 2010 Aug 15. 344(2):603-10. 10.1016/j.ydbio.2010.05.018 PubMed 20493184
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