pBT140
(Plasmid #27493)

• Depositing Lab: Liqun Luo
• Publication: Miyamichi et al Nature. 2010 Dec 22. ():.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha 37 degree in standard LB buffer
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nuclear localization signal-Cre
• Alt name: nls-Cre
• Species: P1 bacteriophage
• Insert Size (bp): 1970
• Tag / Fusion Protein:
  • nuclear localization signal (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: BbsI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Although the kanamycin resistance marker is present in the full plasmid sequence, the depositing lab has never used Kan or Kan/Amp selection for this plasmid. In Addgene's experience, the kanamycin resistance is not functional. According to the Luo lab, the function of this plasmid is not affected.