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Addgene

pmCherry-Cep290
(Plasmid #27380)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27380 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cep290
  • Alt name
    centrosomal protein 290
  • Species
    M. musculus (mouse)
  • Mutation
    please see depositor comments below
  • Entrez Gene
    Cep290 (a.k.a. BC004690, Np, Nphp6, b2b1454C, b2b1454Clo, b2b1752C, b2b1752Clo)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer mCherry-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EGFP from pEGFP-mCep290 was replaced with mCherry. P ease note that during Addgene's QC, we identified mutations L208P, V263A, and K2376R in Cep290. The plasmid should function as reported in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-Cep290 was a gift from Joseph Gleeson (Addgene plasmid # 27380 ; http://n2t.net/addgene:27380 ; RRID:Addgene_27380)

  • For your References section:

    Mutations in CEP290, which encodes a centrosomal protein, cause pleiotropic forms of Joubert syndrome. Valente EM, Silhavy JL, Brancati F, Barrano G, Krishnaswami SR, Castori M, Lancaster MA, Boltshauser E, Boccone L, Al-Gazali L, Fazzi E, Signorini S, Louie CM, Bellacchio E, Bertini E, Dallapiccola B, Gleeson JG. Nat Genet. 2006 Jun . 38(6):623-5. 10.1038/ng1805 PubMed 16682970
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