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Addgene

Vinculin-venus
(Plasmid #27300)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27300 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    3927
  • GenBank ID
    NM_205441
  • Entrez Gene
    VCL (a.k.a. VINC1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus A206K (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Chicken vinculin cDNA was originally isolated by Couto & Craig 1988 PNAS 85: 8535-8539
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Vinculin-venus was a gift from Martin Schwartz (Addgene plasmid # 27300 ; http://n2t.net/addgene:27300 ; RRID:Addgene_27300)

  • For your References section:

    Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA. Nature. 2010 Jul 8. 466(7303):263-6. 10.1038/nature09198 PubMed 20613844
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