pLNCX1 Myr AKT2
(Plasmid
#27294)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27294 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLNCX
- Backbone size w/o insert (bp) 6600
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Vector typeRetroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameprotein kinase B beta
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Alt namePKB beta
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Alt nameakt2
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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MutationPH domain deleted (aa1-107)
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GenBank IDm95936
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Entrez GeneAKT2 (a.k.a. HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA)
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Tags
/ Fusion Proteins
- myristolation sequence (N terminal on insert)
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site StuI (destroyed during cloning)
- 5′ sequencing primer AGC TCG TTT AGT GAA CCG TCA GAT CG
- 3′ sequencing primer ACC TAC AGG TGG GGT CTT TCA TTC CC (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLNCX1 Myr AKT2 was a gift from Morris Birnbaum (Addgene plasmid # 27294 ; http://n2t.net/addgene:27294 ; RRID:Addgene_27294)