pcDNA3.1 AKT3 HA (CT-gfp)
(Plasmid #27293)

• Depositing Lab: Morris Birnbaum
• Publication: Birnbaum lab plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1/CT-GFP-Topo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6157
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: protein kinase B gamma
• Alt name: akt3
• Alt name: Pkb gamma
• Species: M. musculus (mouse)
• Insert Size (bp): 1500
• Mutation: Y473F
• Entrez Gene: Akt3 (a.k.a. AI851531, D930002M15Rik, Nmf350)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstxI (not destroyed)
• 3′ cloning site: BstxI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Edge Biosystems
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GFP is not expressed as there is a stop codon after the HA tag.

mTORC2 reportedly can phosphorylate FSY and FSF sites, so the Y473F mutation should not affect expression or function.