pEF1-alpha-IIb
(Plasmid #27288)

• Depositing Lab: Timothy Springer
• Publication: Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Backbone

• Vector backbone: pEF1/V5-HisA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: integrin alpha IIb
• Alt name: GPIIb, CD41
• Species: H. sapiens (human)
• Insert Size (bp): 3300
• Mutation: n/a
• GenBank ID: NM_000419
• Entrez Gene: ITGA2B (a.k.a. BDPLT16, BDPLT2, CD41, CD41B, GP2B, GPIIb, GT, GT1, GTA, HPA3, PPP1R93)
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Spe I (unknown if destroyed)
• 3′ cloning site: Pme I (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry