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Addgene

pMODloxZeo3DAmp
(Plasmid #27182)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27182 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMOD<MCS>
  • Backbone manufacturer
    Epicentre
  • Backbone size w/o insert (bp) 2000
  • Vector type
    in vitro transposion

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Bla gene is not functional.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lox-EM7p-Zeo-lox cassette flanked by Tn5 outer element
  • Species
    synthetic
  • Insert Size (bp)
    839

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pvu II (not destroyed)
  • 3′ cloning site Pvu II (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that Bleomycin and Zeocin are the same antibiotic.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMODloxZeo3DAmp was a gift from Yoh Wada (Addgene plasmid # 27182 ; http://n2t.net/addgene:27182 ; RRID:Addgene_27182)

  • For your References section:

    Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions. Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y. Nucleic Acids Res. 2005 . 33(5):e52. 10.1093/nar/gni055 PubMed 15784610
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