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Addgene

pGPS21loxFRTNeo
(Plasmid #27180)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGPS21
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 3000
  • Vector type
    in vitro transposion
  • Selectable markers
    Neomycin (select with G418) ; G418 in mouse ES

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    EC100D pir-116 strain
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R
  • Species
    synthetic
  • Insert Size (bp)
    2564

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal I (not destroyed)
  • 3′ cloning site Sal I (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid confers Kan resistance (25ug/ml) and Tet resistance (12.5ug/ml). However, it requires pir factor for replication.

Note that this plasmid is supplied on a stab with only Tet. When re-plating, end users should add Kan as well. Also note that the plasmid is only considered high copy in the pir-116 strain. In a WT pir strain, it is considered low copy.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGPS21loxFRTNeo was a gift from Yoh Wada (Addgene plasmid # 27180 ; http://n2t.net/addgene:27180 ; RRID:Addgene_27180)

  • For your References section:

    Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions. Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y. Nucleic Acids Res. 2005 . 33(5):e52. 10.1093/nar/gni055 PubMed 15784610
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