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Addgene

pEF5B-FRT-GFP-αTAT1
(Plasmid #27099)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27099 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEF5B-FRT-LAP-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5971
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    α-Tubulin K40 acetyltransferase1
  • Alt name
    αTAT1
  • Alt name
    ATAT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    999
  • GenBank ID
    NM_024909
  • Entrez Gene
    ATAT1 (a.k.a. C6orf134, MEC17, Nbla00487, TAT, alpha-TAT, alpha-TAT1)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • TEV site (N terminal on backbone)
    • S-tag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF5B-FRT-GFP-αTAT1 was a gift from Maxence Nachury (Addgene plasmid # 27099 ; http://n2t.net/addgene:27099 ; RRID:Addgene_27099)
  • For your References section:

    The major {alpha}-tubulin K40 acetyltransferase {alpha}TAT1 promotes rapid ciliogenesis and efficient mechanosensation. Shida T, Cueva JG, Xu Z, Goodman MB, Nachury MV. Proc Natl Acad Sci U S A. 2010 Nov 10. ():. 10.1073/pnas.1013728107 PubMed 21068373