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Addgene

pID2.02
(Plasmid #26955)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pie-1
  • Backbone size w/o insert (bp) 17037
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
  • Species
    C. elegans (nematode)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Second Generation pie-1 vector for expressing ORFs in the germline

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pID2.02 was a gift from Geraldine Seydoux (Addgene plasmid # 26955 ; http://n2t.net/addgene:26955 ; RRID:Addgene_26955)

  • For your References section:

    Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos. Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G. Dev Biol. 2008 Nov 1. 323(1):76-87. 10.1016/j.ydbio.2008.07.008 PubMed 18692039
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