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Addgene

-4200GAMT-P1-pGL4
(Plasmid #26804)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26804 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.20
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5404
  • Vector type
    Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Guanidinoacetate N-methyltransferase
  • Alt name
    GAMT promoter (full-length)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4500
  • Entrez Gene
    GAMT (a.k.a. PIG2, TP53I2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer3
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are a few discrepancies between Addgene quality control sequence and NCBI reference sequences, however, these do not affect function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -4200GAMT-P1-pGL4 was a gift from Sam Lee (Addgene plasmid # 26804 ; http://n2t.net/addgene:26804 ; RRID:Addgene_26804)

  • For your References section:

    GAMT, a p53-inducible modulator of apoptosis, is critical for the adaptive response to nutrient stress. Ide T, Brown-Endres L, Chu K, Ongusaha PP, Ohtsuka T, El-Deiry WS, Aaronson SA, Lee SW. Mol Cell. 2009 Nov 13. 36(3):379-92. 10.1016/j.molcel.2009.09.031 PubMed 19917247
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