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Addgene

pShuttle mCherry-Tubulin
(Plasmid #26768)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26768 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pShuttle-CMV
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 7469
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tubulin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_006082
  • Entrez Gene
    TUBA1B (a.k.a. K-ALPHA-1)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F, mCherry-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pShuttle mCherry-Tubulin was a gift from Torsten Wittmann (Addgene plasmid # 26768 ; http://n2t.net/addgene:26768 ; RRID:Addgene_26768)

  • For your References section:

    Analysis of microtubule dynamic instability using a plus-end growth marker. Matov A, Applegate K, Kumar P, Thoma C, Krek W, Danuser G, Wittmann T. Nat Methods. 2010 Sep . 7(9):761-8. 10.1038/nmeth.1493 PubMed 20729842
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