pAdEasy mCherry-Tubulin
(Plasmid #26767)

• Depositing Lab: Torsten Wittmann
• Publication: Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

Backbone

• Vector backbone: pAdEasy-1
• Backbone manufacturer: Stratagene, ATCC
• Backbone size w/o insert (bp): 33414
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tubulin
• Alt name: TUBA1B
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Entrez Gene: TUBA1B (a.k.a. K-ALPHA-1)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F, mCherry-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mCherry-Tubulin was cloned into pShuttle-CMV between KpnI and NotI then recombined into pAdEasy-1

AdEasy is a registered trademark of the Johns Hopkins University.