pDIRE
(Plasmid #26745)

• Depositing Lab: Rolf Zeller
• Publication: Osterwalder et al Nat Methods. 2010 Oct 17

Backbone

• Vector backbone: pPGKFlpobpA
• Backbone manufacturer: Addgene plasmid 13793
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Cre/Lox ; FLP/FRT

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iCre expression cassette
• Species: optimized iCre expression cassette; optimized Flpo expression cassette
• Insert Size (bp): 2500

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PsiI (destroyed during cloning)
• 3′ cloning site: PsiI (destroyed during cloning)
• 5′ sequencing primer: mPGK-F, EF-1a Forward
• 3′ sequencing primer: BGH-rev, SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid has been assembled using PGK-Flpo cassette (Addgene plasmid 13793), iCre (Addgene plasmid 12336) and Ef1alpha promotor (Addgene plasmid 11918)
• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pDIRE plasmid is designed to express the optimized versions of the Cre (iCre) and Flp (Flpo) recombinases and is required for dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521

Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocols/1906