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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4097
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Vector typeMammalian Expression ; reporter for F-actin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUtrophin
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Alt nameUtrCH
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Speciesunknown
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MutationCalponin homology domain of utrophin (aa# 1-261)
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Tag
/ Fusion Protein
- monomeric RFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The calponin homology domain of utrophin (Utr-CH) was fused to monomeric RFP in order to visualize F-actin.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mRFP-UtrCH was a gift from William Bement (Addgene plasmid # 26739 ; http://n2t.net/addgene:26739 ; RRID:Addgene_26739) -
For your References section:
Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin. Burkel BM, von Dassow G, Bement WM. Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32. 10.1002/cm.20226 PubMed 17685442
Map uploaded by the depositor.
