pEGFPc2 mAbp1
(Plasmid #26719)

• Depositing Lab: Anna Huttenlocher
• Publication: Cortesio et al Mol Biol Cell. 2010 Jan 1. 21(1):186-97.

Backbone

• Vector backbone: pEGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Actin-binding Protein-1
• Alt name: mAbp1
• Species: M. musculus (mouse)
• Entrez Gene: Dbnl (a.k.a. RP23-385C16.5, Abp1, SH3P7, mAbp1)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mouse mAbp1 was kindly provided by David Drubin (University of California-Berkeley)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry