pFLAG-Fbw7α-3'UTR
(Plasmid #26650)

• Depositing Lab: Alex Minella
• Publication: Xu et al J Biol Chem. 2010 Sep 7. ():.

Backbone

• Vector backbone: p3XFLAG-Myc-CMV
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fbw7α
• Species: H. sapiens (human)
• Insert Size (bp): 5467
• Tag / Fusion Protein:
  • 3XFLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

3'UTR of FBXW7 immediately follows the stop codon at the end of the FBXW7 reading frame.

C-terminal myc tag present in vector will not be expressed as fusion protein due to FBXW7 stop codon.

Addgene NGS results identified a D705E variant on the C-terminus of FBXW7, which also lacks the final two residues (MK).