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p1553 CMV-EBNA1 (delta GGA)
(Plasmid #26648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pHyg
  • Backbone size w/o insert (bp) 5718
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Epstein-Barr Nuclear Antigen 1
  • Alt name
    EBNA1
  • Alt name
    EBNA-1
  • Species
    Epstein-Barr Virus
  • Insert Size (bp)
    1279
  • Mutation
    deletion of 224 amino acids of EBNA-1 (aa101-324 when compared to P03211.1)
  • GenBank ID
    P03211
  • Entrez Gene
    EBNA-1 (a.k.a. HHV4_EBNA-1.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BssHII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid expresses delta 205 GGA EBNA-1 driven by the CMV IE promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1553 CMV-EBNA1 (delta GGA) was a gift from Bill Sugden (Addgene plasmid # 26648 ; http://n2t.net/addgene:26648 ; RRID:Addgene_26648)

  • For your References section:

    Fusions between Epstein-Barr viral nuclear antigen-1 of Epstein-Barr virus and the large T-antigen of simian virus 40 replicate their cognate origins. Aiyar A, Sugden B. J Biol Chem. 1998 Dec 4. 273(49):33073-81. 10.1074/jbc.273.49.33073 PubMed 9830062
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