pCAG-Cre
(Plasmid #26647)

• Depositing Lab: Anjen Chenn
• Publication: Chenn Lab Plasmids (unpublished)

Backbone

• Vector backbone: pCAG
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cre
• Alt name: Cre-recombinase
• Species: Bacteriophage P1
• Insert Size (bp): 1000

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-Fwd
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cre was excised from pcDNA3Cre as a directionaly sticy blunt by first cutting with HindIII, then blunting with Klenow, and finally cutting with NotI. The directional Cre fragment was cloned into the pCAG backbone. The eGFP cassette was removed from the pCAG-GPF backbone using SmaI (which left a blunt end) and NotI. Cre was then subcloned directionally into the pCAG backbone.

This construct does NOT express GFP.