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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedelta90 beta-catenin
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Alt namebeta catenin
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SpeciesM. musculus (mouse)
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Insert Size (bp)2907
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MutationDeletion of first 90 aa of beta catenin
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Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG_Fwd
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-delta90GFP was a gift from Anjen Chenn (Addgene plasmid # 26645 ; http://n2t.net/addgene:26645 ; RRID:Addgene_26645) -
For your References section:
Persistent expression of stabilized beta-catenin delays maturation of radial glial cells into intermediate progenitors. Wrobel CN, Mutch CA, Swaminathan S, Taketo MM, Chenn A. Dev Biol. 2007 Sep 15. 309(2):285-97. 10.1016/j.ydbio.2007.07.013 PubMed 17706960