flk1 promoter
(Plasmid #26436)

• Depositing Lab: Didier Stainier
• Publication: Jin et al Development. 2005 Dec . 132(23):5199-209.

Backbone

• Vector backbone: pCRII-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3973
• Vector type: Topo cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: flk1 promoter
• Species: D. rerio (zebrafish)
• Insert Size (bp): 7000
• GenBank ID: AY045466

Cloning Information

• Cloning method: TOPO Cloning
• 5′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From the paper:
Approximately 6.5 kb of upstream sequence of the zebrafish flk1 gene (Liao et al., 1997; Thompson et al., 1998) was amplified from wild-type genomic DNA based on the sequence information previously deposited (from –6410 to –3 of the transcriptional start site, GenBank Accession Number AY045466) (Chan et al., 2002), and subcloned into the pCRII TOPO vector (Invitrogen). This fragment has been shown to drive endothelial-specific expression of the green-red coral fluorescent protein (G-RCFP) (Cross et al., 2003). The EcoRI cleaved fragment from this construct was placed 5′ upstream of the enhanced green fluorescent protein (EGFP) vector (Clontech) to drive endothelial specific expression of EGFP